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Fluorescence Filter Sets

Fluorescence Filter Sets for Highly Sensitive Detection of Fluorophores

Materion Balzers Optics fluorescence filter sets include excitation and emission filters (bandpass, shortpass or longpass) as well as a dichroic beamsplitter. Cut-on and cut-off wavelengths of the filters are optimized for the excitation light source and for the fluorescent dye. The filter performance is realized by all-dielectric hard coatings deposited onto a single substrate. Because absorbing glasses and filter mounts are not needed, there is a high flexibility for the filter dimensions in compact optical set-ups.

Fluorescence Filter Sets for Highly Sensitive Detection of Fluorophores

Benefits

High transmittance > 95%
Deep blocking >OD5
Steep filter edges for sharp imaging
Long-term shift-free spectral performance
High environmental stability
High flexibility in filter size

Applications

Fluorescence detection of fluorophores, e.g. CY5, CY3, FITC, TRITC

Technical Data

Excitation and emission filters

Transmittance Tave > 95%

Blocking OD5 /OD6

Angle of incidence AOI=0°

Dimensions standard size Ø 25 mm,
thickness 3 mm (other dimensions on request)

Dichroic Beamsplitter

Transmittance 90% – 97%

Reflectance 90% – 99%

Angle of incidence AOI=45°,
(different AOI on request)

Dimensions standard size 36mmx 26mmx 1mm,
(other dimensions on request)

Substrate fused silica, BK7 or float glas

Parallelism < 3 arcmin

Surface Defects 5 / 3 x 0.1

Environmental Stability Temperature -40 ... + 150 °C
Humidity up to 99%

Schematic of Fluorescence Microscopy

Fig. 1: Measured spectral transmittance in passband (left) and in blockband (right) range.

Fig. 1: Measured spectral transmittance in passband (left) and in blockband (right) range.

Fig.2: Measured spectral transmittance in passband (left) and in blockband (right) range.

Fig.2: Measured spectral transmittance in passband (left) and in blockband (right) range.

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