CorrSlide™

Correlative Microscopy Coverslip, ITO coated, with high precision fiducials

Correlative microscopy is a sophisticated approach combining the imaging capabilities of different, powerful but typically separated microscopy platforms. Laborious, time consuming and limited precision procedures of getting a sample from one instrument to another have been so far one of the biggest struggles in obtaining fast and reliable results. CorrSlide™ technology uses integrated, high precision fiducials which enable easy relocation of samples and finding of exactly the same spot on a sample with micron precision under different resolutions and imaging techniques. Transparent conductive oxide coating (ITO) allows sample investigation under light microscopy and supports charge drain in electron scanning microscopy. It is also compatible with cell cultures and suitable for cells growth. CorrSlide™ can be provided with or without fiducial marks.

Benefits

Transparent for light / fluorescence microscopy
Conductive for scanning electron microscopy
Fiducial marks for precise, manual or automated localization of region of interest
Faster retrieval of region of interest
Secures sample position in relation to fiducials
Enables finding of rare or targeted structures
Enables a retrieval of region of interest even without characteristic points on an image
Enables processing of sample between image acquisitions
Enables integration of imaging platforms
Facilitates merger of functional and structural information
Eliminates need of conductive over-coating for ultrathin samples

Application example: Overlay of light (red) and electron microscopy images allows for classification of neural processes and synapses.

Image courtesy of R. Hahnloser, Institute of Neuroinformatics, University of Zurich and ETH Zurich.

Applications

Light/fluorescence microscopy
Scanning electron microscopy (SEM)
Structured illumination microscopy (SIM)
Bioscience research
Imaging of cells, tissues, soft materials and proteins
Cell biology
Neurobiology
Studies of host-parasite interaction
Analysis of symbiotic relations

Technical Data

Substrate material

D263M glass (colorless borosilicate glass with very low iron content, meets requirements laid down in ISO 8255-1, has high spectral transmission, excellent flatness and a refractive index finely adapted to microscopes, very good resistance to chemical attack, fire polished)
Other materials on request

Substrate dimensions

22mm x 22mm x 0.17 ±0.005mm,

other dimensions and shapes on request

Fiducial marks (customer specific)

High precision
High contrast for excellent visibility
Flexible design adaptable to customer specific application

ITO coating

Premium quality, low defect, full-surface coating
High transparency in VIS range (T_avg>83% λ = 450-780 nm)
Optimized smooth surface texture
Compatible with cell cultures and suitable for cells growth

Other

Durable, unique serial number
Ultraclean surface
Packed under clean-room (class 100) conditions

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